Chromosomal aberrations induced by hydrogen peroxide in cultured mammalian cells.

Abstract

Chromosomal aberrations were observed in cultured mammalian cells (CHO-K1 Chinese hamster cells, V79 Chinese hamster cells, Syrian hamster cells, and BALB/c mouse cells) after treatment with hydrogen peroxide (H2O2; 0.1-0.5mM or 0.01-0.1mM) for 3h. The cytotoxic and clastogenic effects of H2O2 were clearly reduced by the addition of catalase. In contrast to the clastogenic potential, H2O2 did not enhance the frequency of mutation in V79 cells, whether the marker for mutation was 8-azaguanine resistance or ouabain resistance.