Proceedings of the National Academy of Sciences · 1972 · 62 citations · 19 references
Life SciencesGeneticsMicrobiologyEnzymologyMicrobial GeneticsMicrobial MetabolismBiological Nitrogen FixationNitrogen AssimilationNitrogen CycleNitrogen FixationNitrogen MetabolismNitrogen NutritionAmmonium Inhibition Of Nitrogen FixationAzotobacter IsolationMutant PhenotypingNitrogen Fixation GenesNitrogen RegulationNitrogenase ActivityNitrogenase Enzyme SystemNitrogenase Enzymology
Mutants of Azotobacter vinelandii that fix N(2) in the presence of excess NH(4) (+) have been isolated. A mutant that was unable to synthesize component I and component II of nitrogenase was spontaneously reverted to the N(2)-fixing phenotype. Of 21 revertants picked, 7 revertants were not as sensitive as the wild type to repression. A derepressed mutant is as sensitive as the wild type to growth inhibition by 2-methylalanine in the presence of glucose.
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