New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

A comprehensive set of sequence analysis programs for the VAX

John Devereux, Paul Haeberli, Oliver Smithies · Nucleic Acids Research · 1984 · 14.4K citations · Full text

Life Sciences, Bioinformatics, Genetics +8

High efficiency transformation of E.coli by high voltage electroporation

William J. Dower, Jeff F. Miller, Clifton W. Ragsdale · Nucleic Acids Research · 1988 · 2.8K citations · Full text

Life Sciences, Biotechnology, Microbiology +13

Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds

Dominik Mumberg, Rolf Müller, Martin Funk · Gene · 1995 · 2K citations

Life Sciences, Genetics, Molecular Biology +17

A family of low and high copy replicative, integrative and single‐stranded <i>S. cerevisiae</i>/<i>E. coli</i> shuttle vectors

Nathalie Bonneaud, Odile Ozier-Kalogéropoulos, Guo-ya Li et al. · Yeast · 1991 · 527 citations

Life Sciences, Genetics, Molecular Genetics +17