AN EMBEDDING TECHNIQUE FOR ELECTRON MICROSCOPY USING EPON 812

Abstract

Epoxy resins have been established as desirable embedding media for electron microscopy (1-5). Finck (6) described an epoxy resin, EPON 812 (Shell) t, which in addition to its other desirable characteristics, is also appreciably miscible with water. He noted that prolonged alcohol dehydration was not necessary because of the water solubility of EPON 812. We, therefore, considered substitution of EPON 812 for alcohol. Such a procedure u;ould be highly desirable for application of electron microscopy to histochemical investigation in which dehydration by organic solvents may result in considerable removal of lipid and lipidsoluble material from the tissue. This report describes a technique for electron