MORPHOGENETIC STUDIES ON CULTURED MATURE ENDOSPERM OF <i>CROTON BONPLANDIANUM</i>

Abstract

S ummary The mature endosperm of Croton bonplandianum proliferated profusely on White's medium (WM) + 2 ppm 2,4‐D + 5 ppm kinetin + 2500 ppm yeast extract. Association with the embryo was essential for initiation of proliferation of mature endosperm. Tracheidal cells usually differentiated. Twelve‐month‐old callus contained predominantly triploid and hexaploid cells. When the callus obtained on WM + 2,4‐D + kinetin + yeast extract was subcultured on WM triploid roots formed in 25% of the cultures. At 10 −7 M, IBA, IAA, or NAA, in WM, promoted rooting; IBA was most effective. Attempts to induce shoot differentiation failed.