Efficient site-directed mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Life Sciences, Biotechnology, Molecular Biology +10

An efficient one-step site-directed and site-saturation mutagenesis protocol

Lei Zheng · Nucleic Acids Research · 2004 · 1.2K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +15

Reverse vaccinology, a genome-based approach to vaccine development

Rino Rappuoli · Vaccine · 2001 · 493 citations

Life Sciences, Biotechnology, Immunology +13

A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide

Celso R. Ramos, Patrícia Antônia Estima Abreu, Ana L. T. O. Nascimento et al. · Brazilian Journal of Medical and Biological Research · 2004 · 356 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Swine and Poultry Pathogens: the Complete Genome Sequences of Two Strains of<i>Mycoplasma hyopneumoniae</i>and a Strain of<i>Mycoplasma synoviae</i>

Ana Tereza Ribeiro de Vasconcelos, Henrique Bunselmeyer Ferreira, Cristiano Valim Bizarro et al. · Journal of Bacteriology · 2005 · 284 citations · Full text

Life Sciences, Genetics, Microbiology +12