Optics Letters · 2001 · 56 citations · 6 references
Natural SciencesBiomedical EngineeringPhysicsBiomedical OpticsBiophotonicsOpticsFluorescence ImagingFluorescence MicroscopyFourier OpticsNonlinear Optical MicroscopyNonlinear OpticsOptical ImagingAxial ResolutionAxial Resolution ImprovementCutoff FrequencyHarmonic ExcitationHigh-resolution ImagingPoint Spread FunctionReciprocal SpaceResolution Enhancement
A method for increasing lateral as well as axial resolution in fluorescence microscopy is presented. A passband with a high cutoff frequency throughout reciprocal space can be achieved by illumination of the object with spatially harmonic excitation patterns generated by the interference of two collimated laser beams. Theoretical calculations show an almost isotropic point-spread function with a FWHM near 100 nm.
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Reconstructing 3-D light-microscopic images by digital image processing
A. Erhardt, G. Zinser, D. Komitowski et al. · Applied Optics · 1985 · 131 citations