Overexpression of the sterol 14α‐demethylase gene (<i>MgCYP51</i>) in <i>Mycosphaerella graminicola</i> isolates confers a novel azole fungicide sensitivity phenotype

Abstract

The identification of an insertion in the predicted MgCYP51 promoter in azole-resistant isolates overexpressing MgCYP51 is the first report of a genetic mechanism, other than changes in target-site coding sequence, affecting sensitivity to multiple azoles in field isolates of M. graminicola. The identification of recent isolates overexpressing MgCYP51 confirms the ongoing evolution and diversification of resistance mechanisms in European populations of M. graminicola.

References

Page 1

	Year	Citations
Processing of gene expression data generated by quantitative real-time RT-PCR. Patrick Muller, Harald Janovjak, André R. Miserez, PubMed Molecular BiologyTranscriptomics TechnologyReal-time Polymerase Chain ReactionGene Expression ProfilingPolymerase Chain Reaction	2002	1.4K
Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis Stephen B. Goodwin, Sarrah Ben M’Barek, Braham Dhillon, PLoS Genetics	2011	659
Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus Theodore C. White Antimicrobial Agents and Chemotherapy Mrna LevelsImmunologyTotal RnaAntimicrobial ChemotherapyHuman Immunodeficiency Virus	1997	612
A New <i>Aspergillus fumigatus</i> Resistance Mechanism Conferring In Vitro Cross-Resistance to Azole Antifungals Involves a Combination of <i>cyp51A</i> Alterations Emilia Mellado, Guillermo García‐Effrón, Laura Alcázar‐Fuoli, Antimicrobial Agents and Chemotherapy	2007	489
Fungicide-Driven Evolution and Molecular Basis of Multidrug Resistance in Field Populations of the Grey Mould Fungus Botrytis cinerea Matthias Kretschmer, Michaela Leroch, Andreas Mosbach, PLoS Pathogens BiologyField PopulationsAntifungal AgentFungal Cell BiologyMedicine	2009	402
Role of Ascospores in Further Spread of QoI-Resistant Cytochrome <i>b</i> Alleles (G143A) in Field Populations of <i>Mycosphaerella graminicola</i> B. A. Fraaije, H. J. Cools, JM Fountaine, Phytopathology BiologyField PopulationsFungal DiversityEngineeringMolecular Ecology	2005	239
Tandem Repeat of a Transcriptional Enhancer Upstream of the Sterol 14α-Demethylase Gene ( <i>CYP51</i> ) in <i>Penicillium digitatum</i> Hiroshi Hamamoto, Koji Hasegawa, Ryoji Nakaune, Applied and Environmental Microbiology	2000	235
Mutations in the <i>CYP51</i> gene correlated with changes in sensitivity to sterol 14α‐demethylation inhibitors in field isolates of <i>Mycosphaerella graminicola</i> Pierre Leroux, Catherine Albertini, Angélique Gautier, Pest Management Science	2007	220
The 14α-Demethylasse(<i>CYP51A1</i>) Gene is Overexpressed in <i>Venturia inaequalis</i> Strains Resistant to Myclobutanil Guido Schnabel, Alan L. Jones Phytopathology	2001	215
Transcriptional Adaptation of <i>Mycosphaerella graminicola</i> to Programmed Cell Death (PCD) of Its Susceptible Wheat Host J. P. R. Keon, J. F. Antoniw, Raffaella Carzaniga, Molecular Plant-Microbe Interactions EngineeringGeneticsPlant PathologyPlant-pathogen InteractionFungal Developmental Biology	2007	211

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