Genetics and some characteristics of acetate-requiring strains in Neurospora crassa.

Abstract

Ten acetate-requiring mutants of Neurospora crassa were analyzed genetically and examined their growth and enzyme characteristics. It was found that four of the mutants occurred at the known two loci, ace-2 and suc, and the other six were identified as mutations at the new genes, ace-3, ace-4, ace-5 and ace-7. These six acetate genes are scattered all over the linkage map of N. crassa. The ace-2 gene was located between pro-1 and com on the right arm of linkage group III. The ace-3 gene was located between al-1 and nic-1 on the right arm of linkage group I. The ace-4 gene was located between cys-10 and fi on the left arm of linkage group IV. The ace-5 gene was located between am and ure-1 on the right arm of linkage group V. The ace-7 gene was located between nic-2 and cr-1 on the right arm of linkage group I.