Concepedia

Efficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco

Asako Iida, Yasuyuki Yamada, Hiromichi Morikawa

PLANT PHYSIOLOGY · 1991 · 71 citations · 7 references

DOIFull text

Open access

Concepts

Abstract

Plasmid DNA pB1221 harboring beta-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G(2) phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G(1) phases.

References

7

Mitotic protoplasts and their infection with tobacco mosaic virus RNA encapsulated in liposomes

Toshiyuki Nagata, Kazuya Okada, Itaru Takebe · Plant Cell Reports · 1982 · 91 citations

, , +8

Experession and integration of genes introduced into highly synchronized plant protoplasts

Kazuya Okada, Itaru Takebe, Toshiyuki Nagata · Molecular and General Genetics MGG · 1986 · 88 citations

, , +12

Gene delivery into cultured plant cells by DNA-coated gold particles accelerated by a pneumatic particle gun

Aritoshi Iida, Motoaki Seki, Mayumi Kamada et al. · Theoretical and Applied Genetics · 1990 · 75 citations

, , +14

Transient expression of foreign genes in plant cells and tissues obtained by a simple biolistic device (particle-gun)

Hiromichi Morikawa, Asako Iida, Yasuyuki Yamada · Applied Microbiology and Biotechnology · 1989 · 68 citations

, , +16

Evidence That More than 90% of β-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus

Asako Iida, Hiromichi Morikawa · PLANT PHYSIOLOGY · 1991 · 68 citations · Full text

, , +13