DNA Constancy in Heteroploidy and the Stem Line Theory of Tumors

Abstract

Cellular DNA was measured by high-speed flow microfluorometry in mammalian diploid and heteroploid cell populations stained by the fluorescent Feulgen procedure. Heteroploid cells with elevated modal chromosome number showed the expected increase in modal DNA content. However, the variability of DNA content was the same in diploid and heteroploid cell populations despite the large variability of chromosome number in the latter populations. This suggests that heteroploidy may include defects in the chromosomal condensation and kinetochore development systems.