Concepedia

<i>Staphylococcus aureus</i> Cell Wall Stress Stimulon Gene <i>-lacZ</i> Fusion Strains: Potential for Use in Screening for Cell Wall-Active Antimicrobials

Rebecca J. Steidl, Stacy L. Pearson, Robert E. Stephenson, Nagender Ledala, Sutthirat Sitthisak, Brian J. Wilkinson, Radheshyam K. Jayaswal

Antimicrobial Agents and Chemotherapy · 2008 · 27 citations · 23 references

DOIFull text

Open access

Concepts

Abstract

lacZ fusion strains were constructed using the promoters of five cell wall stress stimulon genes: pbp2, tcaA, vraSR, sgtB, and lytR. All fusion strains were induced only in the presence of cell wall-active antibiotics, suggesting the potential of these strains for use in high-throughput screening for new cell wall-active agents.

References

23

Molecular Cloning. A Laboratory Manual

EJ Wood · Biochemical Education · 1983 · 30.6K citations

, , +8

σ<sup>B</sup>Modulates Virulence Determinant Expression and Stress Resistance: Characterization of a Functional<i>rsbU</i>Strain Derived from<i>Staphylococcus aureus</i>8325-4

Malcolm J. Horsburgh, Joanne Aish, Ian White et al. · Journal of Bacteriology · 2002 · 686 citations · Full text

Two‐component system VraSR positively modulates the regulation of cell‐wall biosynthesis pathway in <i>Staphylococcus aureus</i>

Makoto Kuroda, Hiroko Kuroda, Taku Oshima et al. · Molecular Microbiology · 2003 · 513 citations

, , +21

Identification of Critical Staphylococcal Genes Using Conditional Phenotypes Generated by Antisense RNA

Yinduo Ji, Barbara Zhang, Stephanie F. Van et al. · Science · 2001 · 410 citations

, , +15

Epidemic community-associated methicillin-resistant <i>Staphylococcus aureus</i> : Recent clonal expansion and diversification

Adam D. Kennedy, Michaël Otto, Kevin R. Braughton et al. · Proceedings of the National Academy of Sciences · 2008 · 392 citations · Full text

, , +21