Capillary electrophoresis with surface coated capillaries

Abstract

Abstract With uncoated fused silica capillaries, the electroosmotic flow and its contribution to solute migration are in part responsible for problems with the reproducibility of migration time due to hysteresis effects. In transferring coating procedures from silica to capillary surfaces, it has been possible to prepare stable polymeric coatings where hysteresis effects are negligible. Acrylamide coatings reduce the electroosmotic flow. A pH independent flow could be achieved with bonded cation exchange groups. With bonded anion exchangers, reversal of electroosmotic flow is possible.