Proceedings of the National Academy of Sciences · 1979 · 176 citations · 24 references
Life SciencesGeneticsMolecular BiologyMitochondrial BiologyMitochondrial GeneticsNucleic Acid BiochemistryMitochondrial BiochemistryMitochondrial FunctionMitochondrial RibosomesMitochondrial Rna ProcessingMitochondrial StructureMitochondrial TranslationCodon Usage BiasCodon Usage EvolutionMitochondrial Mrna ProcessingMitochondrial Ribosomal ProteinsMitochondrial Ribosomal Rna GenesNucleotide ContentYeast Mitochondrial BiologyYeast Mitochondrial Genetics
We have determined the nucleotide sequence of a segment of Saccharomyces mtDNA that contains the structural gene for one of the subunits (the dicyclohexylcarbodiimide-binding protein) of the mitochondrial ATPase complex. The sequence fits the known amino acid sequence of this protein with the exception of one amino acid. Codon usage is biased in favor of A + T-rich codons. On both sides of the gene, the nucleotide sequence contains less than 4% (mol/mol) G + C for at least 180 nucleotides; these A + T sequences show no evidence of internal repetition. The gene and all the A + T-rich sequence preceding the gene are present in a 12S RNA that is the major transcript of this segment of mtDNA. The nature of the sequences responsible for binding ribosomes to mitochondrial mRNA and for termination of RNA synthesis is considered.
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An insert in the single gene for the large ribosomal RNA in yeast mitochondrial DNA
Johannes L. Bos, Christa Heyting, Piet Borst et al. · Nature · 1978 · 166 citations