Concepedia

Generation and Characterization of a Monoclonal IgG Antibody to Polyethylene Glycol

D. Wunderlich, Margit MacDougall, Diane V. Mierz, Joshuaine G. Toth, Thomas M. Buckholz, Kevin J. Lumb, Haren A. Vasavada

Hybridoma · 2007 · 13 citations · 7 references

DOI

Concepts

Abstract

An IgG mouse monoclonal antibody (10F05) against polyethylene glycol has been generated. The antibody reacts with PEG regardless of the linker used for PEG attachment, and is able to recognize a PEGylated peptide in plasma at concentrations as low as 3 pg/mL. The antibody is readily purified in substantial quantities. The PEG IgG will find significant utility in the sensitive detection of PEG derivitives during the pharmacokinetic characterization of PEGylated compounds.

References

7

Control of hyperuricemia in subjects with refractory gout, and induction of antibody against poly(ethylene glycol) (PEG), in a phase I trial of subcutaneous PEGylated urate oxidase

Nancy J. Ganson, Susan J. Kelly, Edna Scarlett et al. · Arthritis Research & Therapy · 2005 · 343 citations · Full text

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Antibodies against Polyethylene Glycol Produced in Animals by Immunization with Monomethoxy Polyethylene Glycol Modified Proteins

Ary Wolfgang Richter, Eva Åkerblom · International Archives of Allergy and Applied Immunology · 2009 · 282 citations

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Hypersensitivity and Loss of Disease Site Targeting Caused by Antibody Responses to PEGylated Liposomes

Adam D. Judge, Kevin McClintock, Janet R. Phelps et al. · Molecular Therapy · 2005 · 276 citations · Full text

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Accelerated Clearance of Polyethylene Glycol-Modified Proteins by Anti-Polyethylene Glycol IgM

Tian‐Lu Cheng, Pin-Yi Wu, Ming‐Fang Wu et al. · Bioconjugate Chemistry · 1999 · 146 citations

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Repeated injections of PEG-PE liposomes generate anti-PEG antibodies.

Kamila Środa-Pomianek, Janusz Rydlewski, Marek Langner et al. · PubMed · 2005 · 116 citations

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