Essential Oil Composition of<i>Origanum ramonense</i>Danin Leaves from Israel

Abstract

Abstract Essential oils of Origanum ramonense Danin leaves were obtained using two methods: steam-distillation followed by dichloromethane extraction (SDR), and simultaneous purging and extraction (SPE). Volatile constituents of the oils were isolated and identified by GC and GC/MS. A total of 132 compounds were identified using both methods. Among the more than 90 compounds identified in the oil obtained by SDR, 42 were alcohols which composed 79.5% of total GC peak area. The major volatile constituents of this oil were α-terpineol (41.53%), terpinen-4-ol (16.80%), cis-sabinene hydrate (13.17%), eugenol (3.6l%), and γ-terpinene (3.40%). Among 90 constituents identified in oil obtained by SPE, 28 were hydrocarbons which composed 55.70% of total GC peak area. The major constituents of oil obtained by SPE were 1,8-cineol (17.46%), γ-terpinene (14.69%), terpinen-4-ol (13.88%), α-terpinene (9.37%) and α-terpineol (6.83%).