Journal of the American Chemical Society · 2012 · 107 citations · 29 references
Life SciencesBiochemistryBiomolecular EngineeringNanomaterialsPolymer ChemistryVirologyMacromolecular AssemblyProtein StructureProtein-based NanomaterialsSelf-assemblySelf-assembly Of NanomaterialsViral AssemblySelf-assembly MechanismsViral Capsid AssemblyViral Capsid StructureViral Fusion ProteinsVirus Capsid AssemblyVirus-like Particle AssemblyVirus-like Particle Self-assemblyVirus-like Particles
ELP-CP, a structural fusion protein of the thermally responsive elastin-like polypeptide (ELP) and a viral capsid protein (CP), was designed, and its assembly properties were investigated. Interestingly, this protein-based block copolymer could be self-assembled via two mechanisms into two different, well-defined nanocapsules: (1) pH-induced assembly yielded 28 nm virus-like particles, and (2) ELP-induced assembly yielded 18 nm virus-like particles. The latter were a result of the emergent properties of the fusion protein. This work shows the feasibility of creating a self-assembly system with new properties by combining two structural protein elements.
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Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy
Neil P. King, William Sheffler, M.R. Sawaya et al. · Science · 2012 · 676 citations
Viruses: Making Friends with Old Foes
Trevor Douglas, Mark Young · Science · 2006 · 606 citations