Biosynthesis of triglycerides from triose phosphates by microsomes of intestinal mucosa

Abstract

Abstract Microsomes of hamster intestinal mucosa synthesize triglycerides from dihydroxyacetone phosphate or DL‐glyceraldehyde 3‐phosphate in the presence of palmitate, ATP, CoASH and NADPH or NADH but in the absence of soluble enzymes. An inhibitor of triose phosphate isomerase, 1‐hydroxy‐3‐chloro‐2‐propanone phosphate, completely inhibits glyceride synthesis from glyceraldehyde 3‐phosphate. This compound does not inhibit glyceride synthesis from dihydroxyacetone phosphate. These reactions confirm the conversion of glyceraldehyde 3‐phosphate to dihydroxyacetone phosphate prior to glyceride synthesis.