Nitrogenase is restricted to the vesicles in <i>Frankia</i> strain EAN1pec

Abstract

The presence of nitrogenase in vesicles and hyphae of Frankia EAN1pec was investigated by using immunogold labelling on ultrathin cryosections for electron microscopy. These studies resulted in the specific labelling of nitrogenase in the vesicles of nitrogen‐fixing cultures. No significant label could be found in the hyphae, indicating a strong repression of nitrogenase in the hyphae.