A rapid and sensitive method for the determination of ascorbic acid using 4,7-diphenyl-1,10-phenanthroline.

Abstract

The 2,4-dinitrophenylhydrazine (DNP) method has been widely used for the determination of ascorbic acid (AsA) in animal tissues, biological fluids, and foods,1 ~3) but its experimental procedure takes much time and is rather complex. Besides, there might be some possible reaction of carbonyls, especially in biological samples,