Synthesis of [3,5-³H₂-Tyr²³]-β-corticotrophin-(1–24)-tetracosapeptide

Abstract

The synthesis is described of β-corticotrophin-(1–24)-tetracosapeptide labelled with tritium in the tyrosine residue at position 23 to a specific radioactivity of 46 Ci mmol–1 by reductive deiodination of a protected precursor. Evidence for the integrity of the final product is provided by amino-acid analysis, column and thin-layer chromatography, and bioassay, supported by chemical and enzymic analytical data on the protected precursor and the derived tetracosapeptide containing di-iodotyrosine.