Proceedings of the National Academy of Sciences · 2002 · 34 citations · 48 references
Life SciencesImmunologyMolecular BiologyCell SignalingSignal TransductionTranscriptional RegulationImmune Cell SignalingImmune Gene RegulationT Cell ActivationT Cell Activation ControlT Cell BiologyT Cell Receptor SignalingAdaptor Protein Function In T Cell SignalingEstrogen Receptor Fusion SystemsMitogen-activated Protein Kinase CascadesMitogen-activated Protein Kinase Pathway ActivationSignal Transducer And Activator Of Transcription Signaling In T Cell DifferentiationT-cell Factor TranscriptionT-cell Receptor Gene Expression
Shc, a prototypic adapter molecule, has been implicated in T cell receptor (TCR) signal transduction, but its role has not been identified clearly. Here we report that Shc is essential for TCR-induced IL-2 production but is dispensable for CD69 or CD25 expression. Engagement of TCR in mutant Jurkat T cells lacking Shc fails to produce IL-2 because of impaired mitogen-activated protein kinase activation. Activation of c-Rel, a transcription factor essential for IL-2 expression, was impaired also. In contrast, activation of nuclear factor of activated T cell and expression of CD69/CD25 were comparable between the mutant and wild-type Jurkat cells. These defects were rescued by expression of exogenous Shc. Activation of c-Rel using the estrogen receptor fusion protein restored the activation of the IL-2 promoter in an estrogen-dependent manner. These results show that Shc plays an essential role in the TCR-induced activation of c-Rel and the IL-2 promoter.
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Joanne Sloan‐Lancaster, J. Kitchen, Ronald P. Trible et al. · Cell · 1998 · 1.3K citations · Full text
PKC-θ is required for TCR-induced NF-κB activation in mature but not immature T lymphocytes
Zuoming Sun, Chris Arendt, Wilfried Ellmeier et al. · Nature · 2000 · 894 citations