Isolation and cross‐amplification of microsatellites in pink abalone (<i>Haliotis corrugata</i>)

Abstract

Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT)(15) and (CT)(15 ) enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross-amplified in H. corrugata. A set of 12 polymorphic microsatellites were evaluated in a wild population sample (N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy-Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross-amplified in other abalone species, two in H. fulgens, and seven in H. rufescens.