Cloning and Characterization of Cytokeratins 8 and 19 in Adult Rat Striated Muscle

Abstract

We used degenerate primers for the amino- and carboxyl-terminal ends of the rod domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and clone cytokeratins 8 and 19 (K8 and K19) from cardiac muscle of the adult rat. Northern blots showed that K8 has and has in adult cardiac and of the cytokeratins in adult cardiac muscle for K8 and showed the muscle and and domains the and and from of cardiac the cytokeratins and the the experiments that K8 and the cytokeratins the of muscle of the that that cytokeratins in adult to the of the and We used degenerate primers for the amino- and carboxyl-terminal ends of the rod domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and clone cytokeratins 8 and 19 (K8 and K19) from cardiac muscle of the adult rat. Northern blots showed that K8 has and has in adult cardiac and of the cytokeratins in adult cardiac muscle for K8 and showed the muscle and and domains the and and from of cardiac the cytokeratins and the the experiments that K8 and the cytokeratins the of muscle of the that that cytokeratins in adult to the of the and and the of muscle in proteins that the of in the of the of the We the of to in to the that and the of of the and to the of the muscle and has to and the of muscle proteins that the of muscle and the and that and proteins in the the that the the muscle the to the of the the of that of to intermediate that in and in and of proteins that has intermediate filament used intermediate of reverse used intermediate of reverse proteins rod that and of the of the the and of the rod the of muscle intermediate filament and the of muscle and in muscle to the muscle the of and to the of and to of and to to the cytokeratins in and the the in the in the and the of the to the the domains of that the domains of that for the of and in muscle from of and that of the and that the cytokeratins for the of the in adult muscle showed that the cytokeratins domains of the of cytokeratins and the of the identify that in the of degenerate primers to the of ends of the rod for in in the of Northern blots and cytokeratins 8 and 19 (K8 and the cytokeratins in and cardiac of the adult rat. experiments that the cytokeratins the experiments K8 and the of We that K8 and in to the domains and to the and and from adult and adult the from and the in muscle and in of to used for to used for in of to and the for to in and in and used of for in and used of for blots to cytokeratins and 19 from used of for experiments and for to 19 from from and from used in to the for the from used of for in for 19 used of and for the used of and from and to to and to and and from from adult and in to in the in the and in and in for of to to the the in and for of muscle and to and to and and to and in the and of the of the cytokeratins and 19 for in and proteins to and the blots in in and for blots the to the cytokeratins to cardiac muscle from adult of in in for and of the from of to the and to proteins in the of the the of adult cardiac muscle in in of of the in the of in to of the to the and in the for in and proteins from adult and the of used in and to the and in the to the degenerate primers to the of the amino- and carboxyl-terminal ends of the rod domains of intermediate of degenerate primers that and primers to of the cytokeratins to from the 8 19 blots the to the of from adult and and to Northern and of cytokeratins 8 and in the to that to blots to in in the of the Northern blots of the of from of the in the of the and of the to to the from the 8 from primers to the from the of K8 the and of the to 19 in the of the of the in and and in of and the and of the for for for in and in for for the from from that muscle from that to the to the We degenerate primers for in experiments to identify proteins in adult of 8 and 19 from primers to the the and ends of the rod of and primers used in experiments from adult cardiac clone of the rod of 8 We used primers that the for K8 to clone the and of the and from the of the adult the in the and to the the clone used in Northern experiments for K8 from cardiac muscle to the for 8 and to the identify clone in of the cytokeratins of and experiments to identify in adult K8 that to the and to in muscle and muscle We used primers in experiments to in adult We in from adult muscle of from adult to the and to the of for to identify the of in adult the and primers to the to identify the of in from from that cardiac K8 and in adult used of the K8 and in Northern blots to the of for cytokeratins in adult and used for in the Northern in to and and We that in and that the for K8 of of for the in and We of Northern and to in the for K8 in adult and to the of that for the We the of the and of the that for the of the K8 that in the Northern blots for cytokeratins 8 and Northern of from and blots the for cytokeratins 8 and 19 in 8 has of the in and has of that of in the 19 has of in the Northern blots that and muscle of K8 and proteins in the the muscle We used to of the intermediate filament to that the used for cytokeratins and the proteins and to blots the that for the experiments that of to cytokeratins and cytokeratins and 19 and to blots to and that for the the of the in used to the cytokeratins in experiments to cytokeratins in adult muscle the that K8 and the of cytokeratins in adult cardiac to in the We used the to to in cardiac muscle the and for cytokeratins 8 and 19 the and the muscle to that in muscle that of the of the that K8 and the the the and the in the and the of the the and for K8 and of the in cardiac muscle in muscle and in the of to identify of in adult experiments for in showed of the the of the and of cytokeratins 8 and 19 in from adult in and in to and 8 19 and in the for the and and the and cytokeratins the of and for proteins that the cytokeratins the and domains of in to in of and proteins of the We experiments to and to the cytokeratins We of muscle from adult for in to to in cytokeratins of the cytokeratins in from the experiments that to and to and experiments that the cytokeratins and in adult and of and from of adult muscle and from adult muscle to and and to blots in and the that and the of and and the and and to of the and to and to the and of the and of and to the the from of adult cardiac muscle and to the cytokeratins the and and and of the to the and to the of the and in the K8 and that the cytokeratins in to and of proteins in in the for in in the of and to the of in the to the that the proteins used to the of the cytokeratins of that of the to the of the of the of the of the of the and cytokeratins 8 and 19 and (K8 and in that the the of K8 and in the of of K8 in the of the of that of the and that of the the of in the and and the to the that of cytokeratins the that the in experiments and that the of the cytokeratins from to and the to and in that the and for the cytokeratins from that of the of of cytokeratins 8 and 19 of the the of the in and K8 and in in and that from K8 and in the in and that in and of muscle to and to the that in the of and the cytokeratins of from to and to in that in the of from the and and the of cytokeratins the that and the cytokeratins to the and that the of in proteins that the cytokeratins in and muscle of proteins that the of the and to the of and proteins in the in the of proteins the the of the the has the the has that cytokeratins 8 and in adult that the to of the domains of the of in the of the cytokeratins in the of and proteins to the and of the for the of to the of intermediate filament proteins and and of proteins to the in muscle and to in the in the and to of the the of and and of the of proteins in the of the the and proteins in muscle to the of the and proteins the domains of the and the that domains the to of the that for the of in that K8 and in for the and of showed that K8 in adult cardiac and in the for the of in the of the K8 to and of to the for K8 in clone the of the of for the to the for of the in the of K8 and in adult muscle that to that proteins to to the of and to and that in the the intermediate filament proteins the that for the of proteins the that of K8 and the domains of to the of domains in the the domains of in muscle that and the cytokeratins the of and the domains of to the and to to identify the cytokeratins the used of the of cytokeratins that in of the in of showed that the of the and that K8 and the the that the cytokeratins proteins the and domains of the that and in and that cytokeratins experiments in the of that the of and the in We the for from in to showed that K8 and of the that the of the to that the and that cytokeratins for the of the cytokeratins the of the muscle of cytokeratins that to the of of the of the for in in the that K8 and for the in muscle to the the and domains in that of the proteins the cytokeratins the that the cytokeratins the of the and that the of in the to in and the of muscle in proteins that the of in the of the of the We the of to in to the that and the of of the and to the of the muscle and has to and the of muscle proteins that the of muscle and the and that and proteins in the the that the the muscle the to the of the the of that of to intermediate that in and in and of proteins that has intermediate filament used intermediate of reverse used intermediate of reverse proteins rod that and of the of the the and of the rod the of muscle intermediate filament and the of muscle and in muscle to the muscle the of and to the of and to of and to to the cytokeratins in and the the in the in the and the of the to the the domains of that the domains of that for the of and in muscle from of and that of the and that the cytokeratins for the of the in adult muscle showed that the cytokeratins domains of the of cytokeratins and the of the identify that in the of degenerate primers to the of ends of the rod for in in the of Northern blots and cytokeratins 8 and 19 (K8 and the cytokeratins in and cardiac of the adult rat. experiments that the cytokeratins the experiments K8 and the of We that K8 and in to the domains and to the and and from adult and adult the from and the in muscle and in of to used for to used for in of to and the for to in and in and used of for in and used of for blots to cytokeratins and 19 from used of for experiments and for to 19 from from and from used in to the for the from used of for in for 19 used of and for the used of and from and to to and to and and from from adult and in to in the in the and in and in for of to to the the in and for of muscle and to and to and and to and in the and of the of the cytokeratins and 19 for in and proteins to and the blots in in and for blots the to the cytokeratins to cardiac muscle from adult of in in for and of the from of to the and to proteins in the of the the of adult cardiac muscle in in of of the in the of in to of the to the and in the for in and proteins from adult and the of used in and to the and in the to the degenerate primers to the of the amino- and carboxyl-terminal ends of the rod domains of intermediate of degenerate primers that and primers to of the cytokeratins to from the 8 19 blots the to the of from adult and and to Northern and of cytokeratins 8 and in the to that to blots to in in the of the Northern blots of the of from of the in the of the and of the to to the from the 8 from primers to the from the of K8 the and of the to 19 in the of the of the in and and in of and the and of the for for for in and in for for the from and from adult and adult the from and the in muscle and in of to used for to used for in of to and the for to in and in and used of for in and used of for blots to cytokeratins and 19 from used of for experiments and for to 19 from from and from used in to the for the from used of for in for 19 used of and for the used of and from and to to and to and and from from adult and in to in the in the and in and in for of to to the the in and for of muscle and to and to and and to and in the and of the of the cytokeratins and 19 for in and proteins to and the blots in in and for blots the to the cytokeratins to cardiac muscle from adult of in in for and of the from of to the and to proteins in the of the the of adult cardiac muscle in in of of the in the of in to of the to the and in the for in and proteins from adult and the of used in and to the and in the to the degenerate primers to the of the amino- and carboxyl-terminal ends of the rod domains of intermediate of degenerate primers that and primers to of the cytokeratins to from the 8 19 Northern blots the to the of from adult and and to Northern and of cytokeratins 8 and in the to that to blots to in in the of the Northern blots of the of from of the in the of the and of the to to the from the 8 from primers to the from the of K8 the and of the to 19 in the of the of the in and and in of and the and of the for for for in and in for for the from from that muscle from that to the to the We degenerate primers for in experiments to identify proteins in adult of 8 and 19 from primers to the the and ends of the rod of and primers used in experiments from adult cardiac clone of the rod of 8 We used primers that the for K8 to clone the and of the and from the of the adult the in the and to the the clone used in Northern experiments for K8 from cardiac muscle to the for 8 and to the identify clone in of the cytokeratins of and experiments to identify in adult K8 that to the and to in muscle and muscle We used primers in experiments to in adult We in from adult muscle of from adult to the and to the of for to identify the of in adult the and primers to the to identify the of in from from that cardiac K8 and in adult used of the K8 and in Northern blots to the of for cytokeratins in adult and used for in the Northern in to and and We that in and that the for K8 of of for the in and We of Northern and to in the for K8 in adult and to the of that for the We the of the and of the that for the of the K8 that in the the Northern blots that and muscle of K8 and proteins in the the muscle We used to of the intermediate filament to that the used for cytokeratins and the proteins and to blots the that for the experiments that of to cytokeratins and cytokeratins and 19 and to blots to and that for the the of the in used to the cytokeratins in experiments to cytokeratins in adult muscle the that K8 and the of cytokeratins in adult cardiac to in the We used the to to in cardiac muscle the and for cytokeratins 8 and 19 the and the muscle to that in muscle that of the of the that K8 and the the the and the in the and the of the the and for K8 and of the in cardiac muscle in muscle and in the of to identify of in adult experiments for in showed of the the of the and of cytokeratins 8 and 19 in from adult in and in to and 8 19 and in the for the and and the and cytokeratins the of and for proteins that the cytokeratins the and domains of in to in of and proteins of the We experiments to and to the cytokeratins We of muscle from adult for in to to in cytokeratins of the cytokeratins in from the experiments that to and to and experiments that the cytokeratins and in adult and of and from of adult muscle and from adult muscle to and and to blots in and the that and the of and and the and and to of the and to and to the and of the and of and to the the from of adult cardiac muscle and to the cytokeratins the and and and of the to the and to the of the and in the K8 and that the cytokeratins in to and of proteins in in the for in in the of and to the of in the to the that the proteins used to the of the cytokeratins of that of the to the of the of the of the of the of the and cytokeratins 8 and 19 and (K8 and in that the the of K8 and in the of of K8 in the of the of that of the and that of the the of in the and and the to the that of cytokeratins the that the in experiments and that the of the cytokeratins from to and the to and in that the and for the cytokeratins from that of the of of cytokeratins 8 and 19 of the the of the in and K8 and in in and that from K8 and in the in and that in and of muscle to and to the that in the of and the cytokeratins of from to and to in that in the of from the and and the of cytokeratins the that and the cytokeratins to the and that the of in proteins that the cytokeratins in from that muscle from that to the to the We degenerate primers for in experiments to identify proteins in adult of 8 and 19 from primers to the the and ends of the rod of and primers used in experiments from adult cardiac clone of the rod of 8 We used primers that the for K8 to clone the and of the and from the of the adult the in the and to the the clone used in Northern experiments for K8 from cardiac muscle to the for 8 and to the identify clone in of the cytokeratins of and experiments to identify in adult K8 that to the and to in muscle and muscle We used primers in experiments to in adult We in from adult muscle of from adult to the and to the of for to identify the of in adult the and primers to the to identify the of in from from that cardiac K8 and in adult We used of the K8 and in Northern blots to the of for cytokeratins in adult and used for in the Northern in to and and We that in and that the for K8 of of for the in and We of Northern and to in the for K8 in adult and to the of that for the We the of the and of the that for the of the K8 that in the the Northern blots that and muscle of K8 and proteins in the the muscle We used to of the intermediate filament to that the used for cytokeratins and the proteins and to blots the that for the experiments that used to the cytokeratins in experiments to cytokeratins in adult muscle the that K8 and the of cytokeratins in adult cardiac to in the We used the to to in cardiac muscle the and for cytokeratins 8 and 19 the and the muscle to that in muscle that of the of the that K8 and the the the and the in the and the of the the and for K8 and of the in cardiac muscle in muscle and in the of to identify of in adult experiments for in showed of the the of the and that the cytokeratins the and domains of in to in of and proteins of the We experiments to and to the cytokeratins We of muscle from adult for in to to in cytokeratins of the cytokeratins in from the experiments that to and to and experiments that the cytokeratins and in adult the from of adult cardiac muscle and to the cytokeratins the and and and of the to the and to the of the and in the K8 and that the cytokeratins in to and of proteins in in the for in in the of and to the of in the to the that the proteins used to the of the cytokeratins of that of the to the of the of the of the of the of the and cytokeratins 8 and 19 and (K8 and in that the the of K8 and in the of of K8 in the of the of that of the and that of the the of in the and and the to the that of cytokeratins the that the in experiments and that the of the cytokeratins from to and the to and in that the and for the cytokeratins from that of the of of from to and to in that in the of from the and and the of cytokeratins the that and the cytokeratins to the and that the of in proteins that the cytokeratins in and muscle of proteins that the of the and to the of and proteins in the in the of proteins the the of the the has the the has that cytokeratins 8 and in adult that the to of the domains of the the of intermediate filament proteins and and of proteins to the in muscle and to in the in the and to of the the of and and of the of proteins in the of the the and proteins in muscle to the of the and proteins the domains of the and the that domains the to of the that for the of in that K8 and in for the and of showed that K8 in adult cardiac and in the for the of in the of the K8 to and of to the for K8 in clone the of the of for the to the for of the in the of K8 and in adult muscle that to that proteins to to the of and to and that in the the intermediate filament proteins the that for the of proteins the that of K8 and the domains of to the of domains in the the domains of in muscle that and the cytokeratins the of and the domains of to the and to to identify the cytokeratins the used of the of cytokeratins that in of the in of showed that the of the and that K8 and the the that the cytokeratins proteins the and domains of the that and in and that cytokeratins experiments in the of that the of and the in We the for from in to showed that K8 and of the that the of the to that the and that cytokeratins for the of the cytokeratins the of the muscle of cytokeratins that to the of of the of the for in in the that K8 and for the in muscle to the the and domains in that of the proteins the cytokeratins the that the cytokeratins the of the and that the of in the to in and muscle of proteins that the of the and to the of and proteins in the in the of proteins the the of the the has the the has that cytokeratins 8 and in adult that the to of the domains of the the of intermediate filament proteins and and of proteins to the in muscle and to in the in the and to of the the of and and of the of proteins in the of the the and proteins in muscle to the of the and proteins the domains of the and the that domains the to of the that for the of in that K8 and in for the and of Northern showed that K8 in adult cardiac and in the for the of in the of the K8 to and of to the for K8 in clone the of the of for the to the for of the in the of K8 and in adult muscle that to that proteins to to the of and to and that in the the intermediate filament proteins the that for the of proteins the that of K8 and the domains of to the of domains in the the domains of in muscle that and the cytokeratins the of and the domains of to the and to to identify the cytokeratins the used of the of cytokeratins that in of the in of showed that the of the and that K8 and the the that the cytokeratins proteins the and domains of the that and in and that cytokeratins experiments in the of that the We of and the in We the for from in to showed that K8 and of the that the of the to that the and that cytokeratins for the of the cytokeratins the of the muscle of cytokeratins that to the of of the of the for in in the that K8 and for the in muscle to the the and domains in that of the proteins the cytokeratins the that the cytokeratins the of the and that the of in the to in We to for the used in and to for the of