Characterisation of an emerging rickettsia-like organism in Tasmanian farmed Atlantic salmon Salmo salar

Abstract

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 64:37-44 (2005) - doi:10.3354/dao064037 Characterisation of an emerging rickettsia-like organism in Tasmanian farmed Atlantic salmon Salmo salar Serge Corbeil*, Alex D. Hyatt, Mark St. J. Crane AAHL Fish Diseases Laboratory, Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24,Geelong, Victoria 3220, Australia *Email: [e-mail] ABSTRACT: A rickettsia-like organism (RLO) was observed in farmed Atlantic salmon Salmo salar located in south-east Tasmania, Australia. Several assays such as immunoperoxidase, immunoelectron microscopy, polymerase chain reaction and nucleic acid sequencing, as well as phylogenetic analysis of rDNA sequences, were performed on infected fish tissues. Immunohistochemistry results suggested the presence of related antigenic determinants between the Tasmanian RLO and the type strain LF-89 of Piscirickettsia salmonis. However, sequence alignment demonstrated that the Tasmanian RLO contains a 19 bp deletion at the 3'-end of the internal transcribed spacer region of the rDNA operon, indicating a genetic divergence from P. salmonis isolates, which are exotic to Australia. KEY WORDS: Rickettsia-like organism · rDNA sequences · Differential antigenicity · Piscirickettsia salmonis · Atlantic salmon Full article in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 64, No. 1. Online publication date: April 06, 2005 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2005 Inter-Research.