Two interconvertible forms of tryptophanyl sRNA in E. coli.

Abstract

30 The presence of several sRNA components for both leucine and arginine in yeast has been described earlier.Thiebe and Zachau observed two leucyl-and three arginyl-sRNA's after countercurrent distribution in a solvent system different from that used here [Thiebe, R., and H. G. Zachau, Biochim.Biophys.Acta, 103, 568 (1965)].Cherayil and Bock found three distinct peaks for leucyl acceptor activity by chromatography on DEAE columns in the presence of urea [Cherayil, J. D., and R. M. Bock, Biochemistry, 4, 1174 (1965)].Sequence analysis on C14-leucyloligonucleotides obtained after enzymatic digestion of aminoacyl-sRNA gave evidence for the presence of three (or more) different leucyl sRNA's (Herbert, E., personal communication).31 Our colleagues at this University, W. Gartland and N. Sueoka, have independently made an apparently related observation.