High Performance Liquid Chromatographic Determination of Multivitamin Preparations Using a Chemically Bonded Cyclodextrin Stationary Phase

Abstract

Abstract A high performance liquid chromatographic procedure for simultaneous determination of water-soluble vitamin preparations containing pyridoxine hydrochloride (Vit. B6, cyanocobalamin (Vit. B12), thiamine hydrochloride (Vit. B1) and folic acid using a cyclobond I column was developed. The four vitamins were assayed in multivitamin pharmaceutical formulations not containing minerals. Samples were chromatographed using pH 7 phosphate buffer: methanol (80 : 20 %) mobile-phase. The procedure is rapid and the total chromatographic time does not exceed 6.64 min. The method is accurate, precise and specific.