Absorption of Proteins and Peptides in the Far Ultraviolet
Science · 1970 · 79 citations · 8 references
Protein ChemistryUltraviolet LightProtein AssayAbsorption PeakBiochemistryUv-vis SpectroscopyProtein FoldingPeptide LibraryBioanalysisPeptide EngineeringFar UltravioletAbsorption SpectroscopyPeptide BondMedicineBiophysicsProtein Purification
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6 degrees C to take advantage of the reduction of end-absorption of water with temperature.
8
THE FAR ULTRAVIOLET ABSORPTION SPECTRA OF POLYPEPTIDE AND PROTEIN SOLUTIONS AND THEIR DEPENDENCE ON CONFORMATION
Kurt Rosenheck, Paul Doty · Proceedings of the National Academy of Sciences · 1961
404 citations
THE ULTRAVIOLET ABSORPTION SPECTRA OF PROTEINS
Abraham Goldfarb, Leo J. Saidel, Erwin Mosovich · Journal of Biological Chemistry · 1951
211 citations
The spectrophotometric determination of protein at 210mμ
M. P. Tombs, Felicity Souter, N. F. Maclagan · Biochemical Journal · 1959
193 citations
Joe S. Ham, John R. Platt · The Journal of Chemical Physics · 1952
85 citations
THE ABSORPTION SPECTRA OF AMINO ACIDS IN THE REGION TWO HUNDRED TO TWO HUNDRED AND THIRTY MILLIMICRONS
Leo J. Saidel, Abraham Goldfarb, Sheldon. Waldman · Journal of Biological Chemistry · 1952
75 citations