Multiphoton Fluorescence and Second Harmonic Generation Imaging of the Structural Alterations in Keratoconus Ex Vivo

Abstract

MF and SHG microscopy provides three-dimensional structural analysis of keratoconus ex vivo. It may provide important morphologic information for the investigation of the pathogenesis of keratoconus and may have potential in a clinical setting as an in vivo diagnostic and monitoring system for advancing keratoconus.