Isolation and characterization of a cDNA encoding a subtilisin-like serine proteinase (AhSUB) from Acanthamoeba healyi

Basic local alignment search tool

Stephen F. Altschul, Warren Gish, Webb Miller et al. · Journal of Molecular Biology · 1990 · 94.5K citations

Life Sciences, Bioinformatics, Sequence Analysis +3

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

Life Sciences, Molecular Biology, Molecular Genetics +11

Improved tools for biological sequence comparison.

William R. Pearson, David J. Lipman · Proceedings of the National Academy of Sciences · 1988 · 11.3K citations · Full text

Life Sciences, Bioinformatics, Biology +15

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites

Henrik Nielsen, Jacob Engelbrecht, Søren Brunak et al. · Protein Engineering Design and Selection · 1997 · 5.4K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +17

Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

Kun‐Liang Guan, J.E. Dixon · Analytical Biochemistry · 1991 · 1.8K citations

Life Sciences, Biochemistry, Molecular Biology +16