BMB Reports · 2007 · 37 citations · 21 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationGene Expression RegulationGene Regulatory ElementsPromoter ArchitecturePromoter RegulationTranscription FactorsTranscriptional Regulation In EukaryotesGene Promoter RegulationGene Promoter SpecificityGreen Fluorescent Protein ReportersMammalian Expression VectorsMammalian PromotersPromoter ActivationProtein FluorescenceReporter Gene Construction
Many motifs along the 1.2 kb myostatin promoter (MSTNpro) in sheep have been found by the MatInspecter program in our recent study. To further verify the role of the motifs and better understand the transcriptional regulation mechanism of the myostatin gene in sheep, the reporter gene EGFP (enhanced green fluorescent protein) was selected and the wild-type (W) vector MSTNPro(W)-EGFP or motif-mutational (M) vector MSTNPro(M)-EGFP were constructed. The transcriptional regulation activities were analyzed by detecting the fluorescence strength of EGFP in C2C12 myoblasts transfected with the vectors. The results showed that E-box (E) 3, E4, E5 and E7, particularly E3, E5 and E7, had important effects on the activity of the 1.2 kb sheep myostatin promoter. In addition, we also detected several other important motifs such as MTBF (muscle-specific Mt binding factor), MEF2 (myocyte enhancer factor 2), GRE (glucocorticoid response elements) and PRE (progesterone response elements) along the sheep myostatin promoter by the mutational analysis.
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A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle
Luc Grobet, L. J. Royo, Dominique Poncelet et al. · Nature Genetics · 1997 · 1.6K citations