Concepedia

<sup>13</sup>C{<sup>13</sup>C} 2D NMR:  A Novel Strategy for the Study of Paramagnetic Proteins with Slow Electronic Relaxation Rates

Timothy E. Machonkin, William M. Westler, John L. Markley

Journal of the American Chemical Society · 2002 · 82 citations · 8 references

DOI

Concepts

Abstract

Oxidized human [2Fe-2S] ferredoxin has a notably slow electronic relaxation rate, which precludes the observation of signals from nuclei near the iron-sulfur cluster by conventional 2D or 3D methods that utilize proton detection. We have demonstrated the utility of (13)C[(13)C]CT-COSY in identifying connectivity information from fast relaxing carbon nuclei near the paramagnetic center, including those from residues that ligate the metal center.

References

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