Concepedia

A highly specific detection method for diethylstilbestrol in bovine urine by radioimmunoassay following high performance liquid chromatography

Eugène Jansen, R. H. van den Berg, H. van Blitterswijk, R. Both‐Miedema, R.W. Stephany

Food Additives & Contaminants · 1985 · 25 citations · 7 references

DOI

Concepts

Abstract

A specific radioimmunoassay (RIA) for diethylstilbestrol (DES) is presented following purification of sample extracts by isocratic reversed phase high performance liquid chromatography. Applications are given of a forensic investigation for the control of DES in bovine urine. Specificity of HPLC-RIA is compared with that of RIA's with other (chromatographic) purification procedures and with gas chromatography-mass spectrometry. Implications of the use of these techniques in practice and the use of various DES-antisera are discussed.

References

7

Solid-Phase Radioimmunoassay of Estradiol-17β

Guy E. Abraham · The Journal of Clinical Endocrinology & Metabolism · 1969 · 837 citations

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COMPUTER ANALYSIS OF RADIOLIGAND ASSAY AND RADIOIMMUNOASSAY DATA

David Rodbard, J. E. Lewald · European Journal of Endocrinology · 1970 · 612 citations

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Separation and purification of several anabolics present in bovine urine by isocratic high-performance liquid chromatography

Eugène Jansen, R. Both‐Miedema, H. van Blitterswijk et al. · Journal of Chromatography A · 1984 · 29 citations

, , +13

Identification and quantification of diethylstilbestrol in the urine of cattle by both low and high resolution gas chromatography mass spectrometry

H.J.G.M. Derks, J. Freudenthal, J. L. M. Litjens et al. · Journal of Mass Spectrometry · 1983 · 24 citations

, , +16

Identification of several stilbene derivatives in bovine urine by means of high performance liquid chromatographic fractionation and immunochemical detection

Eugène Jansen, R. H. van den Berg, H. van Blitterswijk et al. · Veterinary Quarterly · 1984 · 21 citations

, , +10