Conformational Flexibility in the Peripheral Site of <i>Torpedo c</i><i>alifornica</i> Acetylcholinesterase Revealed by the Complex Structure with a Bifunctional Inhibitor

Abstract

The X-ray crystallographic structure of Torpedo californica acetylcholinesterase (TcAChE) in complex with the bifunctional inhibitor NF595, a potentially new anti-Alzheimer drug, has been solved. For the first time in TcAChE, a major conformational change in the peripheral-site tryptophan residue is observed upon complexation. The observed conformational flexibility highlights the dynamic nature of protein structures and is of importance for structure-based drug design.