Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm

Abstract

A Gram-staining-negative, non-spore-forming, aerobic, non-flagellated, non-gliding, rod-shaped bacterium, designated strain T-y7(T), was isolated from seawater collected in an oyster farm in the South Sea, South Korea, and subjected to a polyphasic study. Strain T-y7(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. In phylogenetic analyses based on 16S rRNA gene sequences, strain T-y7(T) fell within a clade comprising Bizionia species. It formed a coherent cluster with the type strains of Bizionia algoritergicola, B. argentinensis, B. echini and B. myxarmorum, with which it exhibited 16S rRNA gene sequence similarities of 97.4-98.9 % and mean DNA-DNA relatedness values of 11-27 %. Strain T-y7(T) had MK-6 as its predominant menaquinone and iso-C(15 : 1) G, summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), iso-C(15 : 0) and iso-C(17 : 0) 3-OH as its major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The genomic DNA G+C content was 37.1 mol%. Its phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain T-y7(T) does not belong to any established Bizionia species. On the basis of the data presented, strain T-y7(T) is considered to represent a novel species of the genus Bizionia, for which the name Bizionia hallyeonensis sp. nov. is proposed. The type strain is T-y7(T) ( = KCTC 23881(T) = CCUG 62110(T)).