FEMS Microbiology Letters · 2005 · 39 citations · 16 references
AgriculturePlant BiologyPlant SciencesMycologyPlant Molecular BiologySoil MicrobiologyMolecular DiagnosisNucleic Acid ExtractionPathogen IsolationRhizosphere BiologySoil Microbial GeneticsSoil TestingFungal Hyphae IdentificationIsolation From SoilMolecular Identification Of FungiMycelial GrowthPolymerase Chain Reaction (Pcr) AmplificationRestriction Fragment AnalysisSoil Pathogen DetectionSoil Pathogen Diagnostics
Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was >/=11.4 mug of hyphae g(-1) of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.
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