MICROSPECTROPHOTOMETRIC STUDIES ON THE PIGMENTS<i>IN VIVO</i>OF SINGLE ALGAL CELLS‐I. PIGMENTS OF<i>CHLORELLA PYRENOIDOSA</i>

Abstract

Abstract— The pigments in vivo of single cells of Chlorella pyrenoidosa were studied by the microspectrophotometric technique. An accessory recording the first derivative of absorption was used to obtain fine resolution and enhanced accuracy. The results suggest that there are several long‐wavelength components of Chl a in vivo. In addition, there seem to be four short‐wave forms of Chl a. It is also likely that Chl b exists in vivo in two different forms. The existence of all these forms was demonstrated at room temperature.