Metabolism of 1‐¹⁴C‐methyl linoleate hydroperoxide in the rabbit

Abstract

Abstract The metabolism of 1‐ 14 C‐methyl linoleate hydroperoxide (1‐ 14 C‐MLHP) by the rabbit was investigated. Administration of 1.1–1.9 mg of 1‐ 14 C‐MLHP by ear vein injection proved lethal to four of the nine experimental animals. After 2 hr the lungs and liver contained 3.3% and 7.2%, respectively, of the dose. This radioactivity was found to be associated primarily with intact 1‐ 14 C‐MLHP. The triglycerides from these tissues also contained 14 C‐trienoic and 14 C‐dienoic fatty acids. Of the dose, 68% was recovered as 14 CO 2 in 2 hr compared to 39% after 1‐ 14 C‐methyl linoleate injection. The triglycerides from kidney adipose tissue contained a small amount of 14 C‐hydroxy fatty acid, providing confirmation of previous evidence for the presence of a fatty acid hydroperoxide reductase in animal tissues.