A Semisynthetic Strategy to Generate Phosphorylated and Acetylated Histone H2B

Abstract

Synthesis of modified H2B: Semisynthetic modified histone proteins are valuable tools in epigenetic research. Here we describe a novel traceless ligation strategy for histone H2B that provides synthetic access to acetylated and phosphorylated analogues of the protein. These proteins were then applied to biochemical assays to highlight the potential shortcomings inherent in antibody-generated data sets. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.