Nucleosides Nucleotides & Nucleic Acids · 2001 · 11 citations · 2 references
Life SciencesBiotechnologyMicrobiologyBiocatalysisEnzyme TechnologyEnzymologyBiocatalyst EngineeringEnzyme ProductionIndustrial BiocatalysisMicrobial BiocatalysisMicrobial Cell EngineeringWhole-cell BiocatalysisBacterial BiocatalysisBiocatalytic SynthesisEscherichia ColiMicrobial Production Of EnzymesNucleobase SynthesisNucleoside Analog SynthesisNucleoside MetabolismWhole-cell Biocatalyst Engineering
The preparation of nucleosides as well as their base-modified analogues using purified nucleoside phosphorylase enzymes or, more conveniently, using whole bacterial cells is described. The development of genetically modified strains of Escherichia coli, able to over-produce Uridine-phosphorylase and Purine-nucleoside-phosphorylase in the same cells, improves the specific biocatalytic activity and the consequent industrial scale approach.
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