Selection of suitable reference genes for real-time PCR studies of Atlantic halibut development

A new mathematical model for relative quantification in real-time RT-PCR

Michael W. Pfaffl · Nucleic Acids Research · 2001 · 34.3K citations · Full text

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 19.7K citations · Full text

Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

Claus L. Andersen, Jens Ledet Jensen, Torben F. Ørntoft · Cancer Research · 2004 · 7.1K citations

Engineering, Genetics, Transcriptomics Technology +18

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5K citations

Biology, Genetic Analysis, Transcriptional Regulation +13

Guideline to reference gene selection for quantitative real-time PCR

Aleksandar Radonić, Stefanie Thulke, Ian M Mackay et al. · Biochemical and Biophysical Research Communications · 2003 · 1.6K citations

Functional Genomics, Gene Selection, Gene Recognition +10