Nucleic Acids Research · 1982 · 407 citations · 31 references
Life SciencesGeneticsMolecular BiologyGene StructureTranscriptionTranscriptional RegulationCore Promoter ElementsPromoter ArchitecturePromoter RegulationRna StructureTranscription In EukaryotesTranscription Start SitesCaat BoxNucleotide Sequence AlignmentPhosphoglycerate KinasePromoter Sequence DeterminantsPromoter Sequence MotifsYeast Gene Expression RegulationYeast Gene RegulationYeast Promoters
The position of the yeast phosphoglycerate kinase (PGK) gene has been mapped on a 2.95kb Hind III fragment. We have determined the nucleotide sequence of the 5' flanking region and compared this sequence with those from 16 other yeast genes. PGK, like all other yeast genes has an adenine residue at position -3. It has two possible TATA boxes at positions -114 and -152 and a CAAT box at -129. In addition we have defined a structure at position -63 to -39 that is common to all yeast genes that encode an abundant RNA. This structure is a CT-rich block followed, about 10 nucleotides later, by the sequence CAAG.
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