Modern Applied Science · 2009 · 60 citations · 13 references
Life SciencesBiotechnologyMicrobiologyBiocatalysisBiochemical EngineeringBioprocess EngineeringAlcoholic FermentationBiofuel ProductionFermentation KineticsMicrobial BiofuelsMicrobial FermentationYeast BiologyEnzyme ConcentrationEthanol ProductionEthanol Yield OptimizationMichaelis-menten AnalysisOptimum TemperatureRate ConstantsTemperature OptimumYeast Fermentation
Saccharomyces cerevisiae is the cheapest strain available for the conversion of biomass substrate. In the present study, it is used for bio-ethanol production from sugar molasses. The influencing parameters that affect the production of bio-ethanol from sugar molasses are optimized. The optimal values of the parameters such as temperature, pH, substrate concentration, enzyme concentration and fermentation period are found to be 35°C, 4.0, 300 gm/l, 2 gm/l and 72 h respectively. Under this optimum operating condition the maximum of 53% bio-ethanol yield is achieved. The rate of formation of bio-ethanol is found to be well fitted with Michael-Menten equation and the rate constants such as Vmax and Km are found to be 0.71 mol/l sec and 81.63 mol/l respectively.
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