The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line

Abstract

When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10–20% of the cytochrome P‐450‐dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP‐glucuronyltransferase and glutathione‐S‐transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O ‐dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50‐, 30‐ and 10‐fold, respectively.