Biological Chemistry · 2001 · 19 citations · 3 references
Natural SciencesBiophysicsChemistryBiophotonicsBiophysical ChemistrySpectroscopyBiophysical SpectroscopyFluorescence SpectroscopyOptical SpectroscopyPhotophysics Of Fluorescent ProbesSpectroscopy Of BiomoleculesFluorescence Correlation SpectroscopyFluorescence Quantum YieldFluorescent Dye LabelingFluorescent DyesFluorescent LabelingFluorescent LabelsLasersProtein Fluorescence
Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.
3