Comparison of PROSPECT and HPLC estimates of leaf chlorophyll contents in a grapevine stress study

Abstract

This letter describes how PROSPECT, updated with a 1 nm chlorophyll absorbance database, was used to infer total chlorophyll content from the measured reflectance spectra of grapevine leaves. Modelled and measured reflectance spectra agreed on average to within 13% over the range 400–1600 nm. However, discrepancies between modelled and measured reflectances of up to 40% were observed in the spectral region dominated by chlorophyll content. In terms of estimating total chlorophyll content, PROSPECT gave estimates that were on average within 10 µg cm−2 of those obtained using high performance liquid chromatography.