Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter

Abstract

A Gram-negative, catalase-negative, oxidase-positive, rod-shaped bacterium, strain DQHS21(T), was isolated from sediment of a seawater pond used for sea cucumber culture at Jimo in Qingdao province on the east coast of China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DQHS21(T) belonged to the genus Cohaesibacter, sharing the highest sequence similarity (96.1 %) with Cohaesibacter gelatinilyticus CL-GR15(T), while the similarity to other strains was below 93.0 %. The cellular fatty acids consisted mainly of C(18 : 1)ω7c (60.7 %), C(18 : 0) (17.8 %), C(16 : 0) (8.5 %) and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 6.0 %), which together accounted for 93 % of the total fatty acids. Ubiquinone 10 was the major quinone. The G+C content of the chromosomal DNA of strain DQHS21(T) was 55.2 mol%. The combined genotypic and phenotypic data showed that strain DQHS21(T) represents a novel species of the genus Cohaesibacter, for which the name Cohaesibacter marisflavi sp. nov. is proposed, with the type strain DQHS21(T) ( = CGMCC 1.9157(T) = NCCB 100300(T)).