Activation of T Cells from the Intestinal Lamina Propria of the Pig

Abstract

This study found CD4+ T cells present in leucocyte populations isolated from the lamina propria of the pig to be almost exclusively CD45RC-, consistent with their being highly differentiated by exposure to antigen. Following activation in vitro these cells up-regulated expression of IL-2R with similar kinetics to splenic CD4+ cells. However, while splenic cells progressively secreted IL-2 into cultures during the first 24 h, IL-2 was not detected in supernatants of lamina propria cells after 8 h. Reverse-transcriptase polymerase chain reaction (RT-PCR) confirmed that this reflected a transcriptional difference: IL-2 transcripts were detected in cultures of splenic and lamina propria cells in the first few hours after activation but persisted only in splenic cells. In contrast, IL-4 transcripts were strongly expressed by activated lamina propria cells. Cell-cycle analysis demonstrated that fewer lamina propria CD4+ cells progressed into S-phase than did splenic CD4+ cells (26.0 11.1% and 45.0 11.3% respectively, P=0.011). Our results suggest that CD4+ T cells in these populations are differentiated effector cells whose potential for expansion may be dependent upon local factors. Such cells may be targets for immunoregulation by their local microenvironment.