Soft Lithographic Patterning of Hyaluronic Acid on Hydrophilic Substrates Using Molding and Printing

Abstract

Hyaluronic acid (HA) was patterned using two soft lithographic methods on various substrates including glass, silicon dioxides, poly(hydroxyethyl methacrylate) [poly(HEMA)], polystyrene cell culture dishes, and biodegradable polylactic glycolic acid (PLGA), with no or minor chemical modification. Results indicate that HA could be used as a general platform on hydrophilic substrates for cell and protein patterning (see Figure).