Separation Science and Technology · 1995 · 11 citations · 18 references
Natural SciencesBiochemistryChemistryComputational ChemistryProtein BiochemistryTheoretical ChemistryAmino Acid ChemistryAmino Acid PropertiesAqueous Two-phase SystemsPartition CoefficientPhase EquilibriaSolvation ModelingAmino AcidsAqueous Phase PartitioningAqueous Two-phase PartitioningBiomolecule PartitioningHydrophobic Effects On PartitioningHydrophobicityPh Effects On PartitioningPh-dependent Partitioning
Abstract A mathematical model incorporating both the influence of pH and solute hydrophobicity is presented to describe the partitioning of charged compounds in aqueous two-phase systems. The model is applied to the partitioning of amino acids in PEG/potassium phosphate aqueous two-phase systems in which the pH lies between 6.1 and 11.9. The model predicts the minimum in the partition coefficient of several amino acids which is observed in phase systems of intermediate pH. Key Words: Poly(ethylene glycol)PurificationPotassium phosphatePartitioningChargeAmino acids
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Partition of Cell Particles and Macromolecules
Journal of Chromatography A · 1973 · 2K citations
Effects of Salts on the Partition of Proteins in Aqueous Polymeric Biphasic Systems.
Göte Johansson, Annikki Sarnesto, ERIK HUGE‐JENSEN et al. · Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry · 1974 · 156 citations · Full text